Device

Part:BBa_K598026:Design

Designed by: Shuobing CHEN, Tong MU   Group: iGEM11_pku_riboclamp   (2011-09-28)

AND gate (BBa_K228258) regulated by TPP riboswitch 1.20 (BBa_K598004)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2144
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1976
    Illegal AgeI site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1323


Design Notes

The RBS of the TPP ribozyme is the native one.


Source

The AND gate we utilized was designed by Anderson and his colleagues and consequently standardly redesigned by PKU_Beijing 09 team.

References